A high-throughput, configurable pipeline for predicting putative vaccine candidates in prokaryotic pathogens using subtractive reverse vaccinology. Takes a FASTA proteome as input and delivers ranked candidates with B-cell and T-cell epitope annotations.
VacSol automates high-throughput in silico vaccine candidate prediction by integrating established bioinformatics algorithms into a single, multi-mode pipeline. It screens entire bacterial proteomes to rapidly shortlist putative vaccine candidates (PVCs).
Accepts full bacterial proteomes in FASTA format and screens all proteins through a sequential, configurable filtering pipeline.
Identifies extracellular and surface-exposed proteins — the primary accessibility filter for viable vaccine targets.
Removes proteins homologous to the human host proteome, minimizing cross-reactivity risk and false positive candidates.
Prioritizes proteins identified as essential and virulent, targeting proteins that are critical for pathogen survival
Retains only proteins with fewer than two transmembrane helices, ensuring target accessibility for immune recognition.
Predicts antigenic B-cell and T-cell epitopes on shortlisted PVCs. Results exported in five output formats.
Supports both a user-friendly GUI mode and a standalone command-line mode for batch processing and automation.
Validated and compared against published data using the <em>Helicobacter pylori</em> 26695 reference strain as benchmark.
Submit your bacterial proteome FASTA file and VacSol processes it.
Provide the complete bacterial proteome in FASTA format. VacSol accepts input via file path, GUI upload, UniProt ID, or NCBI accession number.
Format: .fasta / .faa
Predicts subcellular localization and retains only secreted proteins and those residing in the outer membrane with surface exposure.
Tool: Localizer module
BLASTs remaining candidates against the human proteome. Proteins with significant similarity are discarded to reduce autoimmune risk.
Tool: Blaster module
Retains proteins with ≤ 2 transmembrane helices. Proteins with excessive membrane-spanning regions are removed as poor vaccine candidates.
Tool: Helicer module
Predicts B-cell and T-cell epitopes on final PVCs. Results are generated in five output formats including complete reports per candidate.
Tool: Epitoper module
VacSol can be installed on Windows or Linux by following these simple steps.
Windows — VacSol.jar
✓ Requires Java Runtime Environment (JRE)
Linux (Ubuntu) – Bvac_deb.deb
$ sudo apt install default-jre
# 3. Run the application
$ java -jar VacSol.jar
All plans are currently free. Pricing will be announced soon.
0$ / forever
Full access · No credit card needed
100$ / year
Advanced features for labs & institutions
Features coming soon. Stay tuned for updates.
Free to use. No license required. Choose your platform below.
Step-by-step setup & usage instructions
Reference data for DEG essential gene codes
If VacSol contributed to your research, please cite the original publication.
PMID: 28193166
PMC5307925
DOI: 10.1186/s12859-017-1540-0
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