Expertly analyzes protein sequence data, providing unparalleled insights for the development of targeted vaccines against a wider spectrum of resistant bacteria.
Bvac-AI uses advanced algorithms to analyze the proteome sequences of bacterial pathogens and identify potential vaccine antigens.
Identifies surface-exposed and secreted proteins — the first and most critical filter for accessible vaccine targets.
Removes proteins with significant similarity to human proteins, minimizing the risk of cross-reactivity.
Cross-references candidates against a built-in virulence factor database to prioritize pathogenically relevant proteins.
Predicts MHC-I and MHC-II binding epitopes for shortlisted candidates using built-in epitope libraries.
Compiled with PyInstaller v6.10.0 runs as a standalone executable on Windows and Linux (Ubuntu). No Python installation, internet connection, or command-line knowledge required.
Users can fine-tune reliability scores, identity percentage thresholds, epitope lengths, and filter selections directly through the GUI to suit their specific organism and analysis goals.
The pipeline takes protein/proteome sequences in fasta as input. The state-of-the-art immunoinformatic tools are integrated into a streamlined computational pipeline
Upload your bacterial protein FASTA file (.faa or .fasta) via the GUI and save your session to a user-defined output directory.
Format: .faa / .fasta
Retains secreted, outer membrane, and fimbrial proteins using pre-saved LocTree3 datasets. Select bacterial genus and strain.
Reliability ≥ 50 · Identity ≥ 70%
Removes proteins with similarity to the human reference proteome (UniProt) to minimize the risk of autoimmune cross-reactions.
Identity 35% · Non-homology 70%
Screens remaining candidates against 27,502 VFDB entries to identify proteins essential to the pathogen’s virulence.
Identity ≥ 70%
Maps B-cell, CD4+, and CD8+ T-cell epitopes on final candidates. Results and FASTA files saved to the output directory.
All lengths · Identity ≥ 50%
B-vac AI can be installed in Windows or Linux by simple steps
Windows – Bvac_Setup.exe
# 1. Download Bvac Setup.exe from mgbio.tech/tools/
# 2. Double-click to run the installer
# 3. Follow the setup wizard
# 4. Launch B-vac from Desktop or Start Menu
✓ No Python or terminal required
Linux (Ubuntu) – Bvac_deb.deb
# 1. Copy Bvac_deb.deb to your home directory
# 2. Install the package
$ dpkg-deb –build
# 3. Run the application
$ B-vac
✓ Tested on Ubuntu 22.04.2 LTS
All plans are currently free. Pricing will be announced soon.
0$ / forever
Full access · No credit card needed
100$ / year
Advanced features for labs & institutions
Features coming soon. Stay tuned for updates.
COMING SOON
If B-vac contributed to your research, please cite the original publication.
PMC12394703
AMA: Ali A, Hamid MHB, Nasir S, Ishaq Z, Anwer F. B-vac a robust software package for bacterial vaccine design. Sci Rep. 2025;15:31745.
APA: Ali, A., Hamid, M. H. B., Nasir, S., Ishaq, Z., & Anwer, F. (2025). B-vac a robust software package for bacterial vaccine design. Scientific Reports, 15, 31745.
MLA: Ali, Amjad, et al. “B-vac a Robust Software Package for Bacterial Vaccine Design.” Scientific Reports, vol. 15, 2025, p. 31745.
NLM: Ali A, Hamid MHB, Nasir S, Ishaq Z, Anwer F. B-vac a robust software package for bacterial vaccine design. Sci Rep. 2025 Aug 28;15:31745. PMCID: PMC12394703.
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