B-Vac is a robust, standalone software package for bacterial vaccine design using reverse vaccinology. B-vac features a user-friendly GUI, runs fully offline, and requires no external libraries or command-line expertise.
B-vac integrates four internally designed filtering modules into a single executable pipeline. It uses a string-based matching approach to compare user-provided proteomes against a curated built-in database — no internet or external dependencies needed.
Identifies surface-exposed and secreted proteins — the first and most critical filter for accessible vaccine targets.
Removes proteins with significant similarity to human proteins, minimizing the risk of cross-reactivity.
Cross-references candidates against a built-in virulence factor database to prioritize pathogenically relevant proteins.
Predicts MHC-I and MHC-II binding epitopes for shortlisted candidates using built-in epitope libraries.
Compiled with PyInstaller v6.10.0 runs as a standalone executable on Windows and Linux (Ubuntu). No Python installation, internet connection, or command-line knowledge required.
Users can fine-tune reliability scores, identity percentage thresholds, epitope lengths, and filter selections directly through the GUI to suit their specific organism and analysis goals.
Upload your proteome FASTA file, set your thresholds, and B-vac handles the rest through its GUI.
Upload your bacterial protein FASTA file (.faa or .fasta) via the GUI and save your session to a user-defined output directory.
Format: .faa / .fasta
Retains secreted, outer membrane, and fimbrial proteins using pre-saved LocTree3 datasets. Select bacterial genus and strain.
Reliability ≥ 50 · Identity ≥ 70%
Removes proteins with similarity to the human reference proteome (UniProt) to minimize the risk of autoimmune cross-reactions.
Identity 35% · Non-homology 70%
Screens remaining candidates against 27,502 VFDB entries to identify proteins essential to the pathogen’s virulence.
Identity ≥ 70%
Maps B-cell, CD4+, and CD8+ T-cell epitopes on final candidates. Results and FASTA files saved to the output directory.
All lengths · Identity ≥ 50%
B-vac can be installed in Windows or Linux by simple steps
Windows – Bvac_Setup.exe
# 1. Download Bvac Setup.exe from mgbio.tech/tools/
# 2. Double-click to run the installer
# 3. Follow the setup wizard
# 4. Launch B-vac from Desktop or Start Menu
✓ No Python or terminal required
Linux (Ubuntu) – Bvac_deb.deb
# 1. Copy Bvac_deb.deb to your home directory
# 2. Install the package
$ dpkg-deb –build
# 3. Run the application
$ B-vac
✓ Tested on Ubuntu 22.04.2 LTS
All plans are currently free. Pricing will be announced soon.
0$ / forever
Full access · No credit card needed
100$ / year
Advanced features for labs & institutions
Features coming soon. Stay tuned for updates.
If B-vac contributed to your research, please cite the original publication.
PMC12394703
AMA: Ali A, Hamid MHB, Nasir S, Ishaq Z, Anwer F. B-vac a robust software package for bacterial vaccine design. Sci Rep. 2025;15:31745.
APA: Ali, A., Hamid, M. H. B., Nasir, S., Ishaq, Z., & Anwer, F. (2025). B-vac a robust software package for bacterial vaccine design. Scientific Reports, 15, 31745.
MLA: Ali, Amjad, et al. “B-vac a Robust Software Package for Bacterial Vaccine Design.” Scientific Reports, vol. 15, 2025, p. 31745.
NLM: Ali A, Hamid MHB, Nasir S, Ishaq Z, Anwer F. B-vac a robust software package for bacterial vaccine design. Sci Rep. 2025 Aug 28;15:31745. PMCID: PMC12394703.
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