B-PAN is the updated version of PanRV, a software package for robust pan-genome analysis. B-Pan integrates functional annotations and algorithms for phylogenetic and evolutionary analysis
B-Pan is a comprehensive bioinformatics tool for full Pan-Genome analysis. It performs pan-genome construction, pan-based phylogenetic tree construction, and functional annotations including COG and KEGG analysis, all within a single, streamlined workflow
Accepts GFF-annotated genome files from multiple isolates of the same species, all pre-annotated with Prokka 1.12 for consistent annotation
Integrates Roary for rapid large-scale pangenome analysis, classifying all genes into pan, core, accessory, and unique gene families across all isolates
Applies six sequential sub-filters — localization, essentiality, virulence, homology, transmembrane helices, and molecular weight — to shortlist PVCs
Prioritizes proteins identified as essential (DEG database) and virulent (VFDB/MvirDB), targeting factors critical for pathogen survival and infectivity
Retains only proteins with fewer than two transmembrane helices via HMMTOP v2.0, ensuring purifiability and immune accessibility of candidates
Predicts B-cell (ABCPred), MHC class-I (ProPred1), and MHC class-II (ProPred) epitopes. VaxiJen v2.0 confirms antigenicity (threshold >0.4)
FAM reveals biological significance via UniProt/COG. ARM screens PVCs against CARD database to detect antibiotic resistance associations
Tested on 301 S. aureus strains. Pangenome: 11,384 pan / 1,524 core / 6,793 accessory / 3,067 unique genes. 7 core-genome PVCs identified. Max runtime: 5h 35min on a 4-core system
Submit your annotated genome files and B-Pan processes them
Multiple GFF-annotated files from all isolates of the target species. All genomes annotated via Prokka 1.12 for consistency.
Roary estimates the pangenome. An in-house bash script generates pan, core, accessory, and unique gene categories in protein FASTA format.
Selected pangenome category passes through six sequential sub-filters. Survivors are classified as putative vaccine candidates (PVCs).
PVCs are subjected to B-cell and T-cell epitope prediction. Epitopes with VaxiJen score >0.4 are retained as potent antigenic candidates.
Functional significance determined via UniProt and COG 2014 BLAST searches with user-defined thresholds set in GUI.
CARD-based screening detects resistance associations. ARM can be run before RVM to pre-filter for anti-AMR vaccine discovery.
B-Pan can be installed on Ubuntu Linux using the provided automated installer
Windows – Bvac_Setup.exe
# 1. Download Bpan_Setup.exe from mgbio.tech/tools/
# 2. Double-click to run the installer
# 3. Follow the setup wizard
# 4. Launch B-pan from Desktop or Start Menu
✓ No Python or terminal required
Linux (Ubuntu) – Bvac_deb.deb
# 1. Copy Bpan_deb.deb to your home directory
# 2. Install the package
$ dpkg_1 Bpan_deb.deb
# 3. Run the application
$ B-pan
✓ Tested on Ubuntu 22.04.2 LTS
All plans are currently free. Pricing will be announced soon.
0$ / forever
Full access · No credit card needed
100$ / year
Advanced features for labs & institutions
Features coming soon. Stay tuned for updates.
COMING SOON!
If B-Pan contributed to your research, please cite the original publication.
PMID: 30871454
PMC6419457
DOI: 10.1186/s12859-019-2713-9
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