Comprehensive molecular biology solutions for nucleic acid isolation, gene expression, cloning, and protein engineering.
We offer high-quality DNA and RNA extraction services from a variety of biological sources, ensuring high yield, purity, and integrity suitable for downstream applications. Our RNA samples are DNase-treated to eliminate genomic contamination. Additionally, we provide reliable cDNA synthesis using reverse transcription protocols optimized for gene expression studies, qPCR, and transcriptomic analysis.
Our team designs primers tailored to your specific applications, including conventional PCR, real-time PCR, cloning, mutagenesis, and sequencing. We ensure optimal melting temperature, specificity, GC content, and minimal secondary structures using advanced design tools and in silico validation.
We provide optimized PCR setups for amplifying target DNA sequences across a wide range of templates. This service supports genotyping, mutation analysis, and routine molecular diagnostics with high accuracy and reproducibility.
Our real-time PCR service enables accurate gene expression analysis and DNA quantification using SYBR Green and probe-based detection systems like TaqMan. We offer assay optimization, standard curve generation, and complete data analysis to support precise and publication-ready results.
Our cloning services include amplification, restriction digestion, ligation, and transformation into competent cells. We support blunt-end, TA, and directional cloning for plasmid construction and downstream applications.
We perform targeted nucleotide changes within a gene to study protein function, improve enzyme activity, or alter gene regulation. Our approach ensures high efficiency and sequence accuracy.
We design and generate recombinant constructs for overexpression in bacterial, yeast, or mammalian systems. This includes tag integration (e.g., His-tag, FLAG), codon optimization, and expression testing to support protein purification and functional studies.